• critical thinking & problem solving – the most applicable skill I gained from my Ph.D.!
• cryo-EM single particle analysis
• X-ray crystallography
• protein expression and purification using liquid chromatography including AKTA Pure
• molecular biology techniques – construct design & cloning
• binding assays – ELISA, SPR, BLI
• structure determination & analysis
• antibody sequence & structural analysis
• antibody engineering
• scientific communication – manuscript preparation & oral presentation
• interpersonal communication – formal & informal mentorship, project direction negotiation, keeping clear records

more details:

• independent data collection on Glacios, Krios, & Arctica TEMs using both SerialEM & EPU
• cryo-EM data processing using cryoSPARC & RELION in Linux and cluster environments
• use of manual and automated (Mosquito & Oryx8) methods for protein crystallization trials
• X-ray crystallography data collection & processing using Blu-Ice, XDS, & Phaser
• model building – Phenix, Coot, AlphaFold
• structural analysis & figure making – ChimeraX, PyMol, Adobe Illustrator
• PDB & EMDB Deposition